Journal: Frontiers in Bioengineering and Biotechnology
Article Title: Design of Hydrogel Silk-Based Microarrays and Molecular Beacons for Reagentless Point-of-Care Diagnostics
doi: 10.3389/fbioe.2022.881679
Figure Lengend Snippet: Effect of the percentage of GC content on the hybridization kinetics of the MB-target. Mean ± standard deviation from 6 independent microarrays measuring the mean fluorescence from at least 6 spots for each microarray. Three MBs with different stems were assayed consisting of 40 (blue), 60 (red) and 100 (fuchsia) % GC content in their stem. Arrow indicates the time of target application to the microarrays. Mean fluorescence was obtained from regions of interest (ROI) using the plot profile function from ImageJ for each microarray spot and measured overtime (see ). Then, means from all spots measured overtime were used to calculate the final mean ± standard deviation (see ).
Article Snippet: All microarray data were analyzed using the ImageJ line profile tool and Igor Pro v7 (WaveMetrics, Portland, OR, United States).
Techniques: Hybridization, Standard Deviation, Fluorescence, Microarray